Clinical Psychopharmacology and Neuroscience Papers in Press available online.

Relationship of circadian rhythm in behavioral characteristics and lipid peroxidation of brain tissues in mice
Chi Eun Oh 1, Hyun Ju Lim 2, Jeonghyun Park 2, Eunsoo Moon 2,3,*, Ji Kyoung Park 4
1Department of Pediatrics, Kosin University College of Medicine, Busan, Korea, 2Department of Psychiatry and Medical Research Institute, Pusan National University Hospital, Busan, Korea, 3Department of Psychiatry, Pusan National University College of Medicine, Yangsan, Korea, 4Department of Pediatrics, Inje University College of Medicine, Busan Paik Hospital, Busan, Korea
Objectives: This study aimed to explore the relationship among several indices of circadian rhythms and lipid peroxidation of brain tissue in mice.
Methods: After entrainment of 4-week-old mice, one group was disrupted their circadian rhythms for three days and the other group for seven days (n=10, respectively). After a recovery period, the Y-maze test, the elevated plus maze test, the tail suspension test, and the forced swimming test were conducted. To assess lipid peroxidation in brain tissue, thiobarbituric acid reactive substances were measured in the cortex, hippocampus, and cerebellum.
Results: When circadian rhythms were disrupted and adapted back to their original rhythm, the recovery time of the 7-day disruption group (median 3.35 days) was significiantly faster than one of the 3-day disruption group (median 4.87 days). In the group with a 7-day disruption, mice that had recovered their rhythms early had higher malondialdehyde levels in their hippocampus compared to those with delayed recovery. The entrainment of circadian rhythms was negatively correlated with the malondialdehyde level of brain tissue. The behavioral test results showed no differences depending on the disruption durations or recovery patterns of circadian rhythms.
Conclusions: These results suggest that disruption types, recovery patterns, and the entrainment of circadian rhythms are likely to affect oxidative stress in adolescents or young adult mice. Future study is needed to confirm and specify these results on the effects of circadian rhythms on oxidative stress and age-dependent effects.
Accepted Manuscript [Submitted on 2021-04-13, Accepted on 2021-08-11]