Fig. 3. Effect of different ligands on tubulin polymerization in vitro. Purified microtubule polymerization kinetics in PEM buffer, in the presence of 1-mM GTP of the Con, Uns, and Sus groups.
Con, control; PEM, 80 mM PIPES, pH 6.9, 0.5 mM EGTA, 2 mM MgCl2; Sus, susceptible; Uns, unsusceptible; GTP, guanosine triphosphate; OD, optical density.
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