Are MASP-2 and AMBP the potential biomarkers of manic episode ? A study via urinary proetomic analysis.
Cem Cerit 1*, Mehmet Sarıhan 2, Ömer Nart 3, Murat Kasap 2, Hilmi Yaşar 1, Gürler Akpınar 2
1Kocaeli University, School of Medicine, Department of Psychiatry, 2Kocaeli University, School of Medicine, Department of Medical Biology, 3Bursa State Hospital, Clinic of Psychiatry
Received: June 10, 2020; Revised: August 15, 2020; Accepted: August 28, 2020; Published online: August 28, 2020.
© The Korean College of Neuropsychopharmacology. All rights reserved.

Abstract
Objective: Investigating the molecular basis of Bipolar Disorder (BD) is crucial in terms of developing effective treatment strategies as well as objective laboratory-based diagnostic tools for the disease.
Methods: In this study, we examined the urine samples of BD patients both in manic episode and after remission and compared their urinary protein profiles with the healthy controls. Twelve patients and twelve controls (C group) included to the study. Urinary samples of patients were first collected during manic episode (M group) and then after remission (R group). 2-DE coupled to MALDI-TOF/TOF approach was used to assess the difference in proteome profiles of the groups. Western blot analysis was used for verification of 2-DE results.
Results: AMBP, MASP-2 and lgG-1-chain displayed significant increases in their abundance in the urine protein pool of M group in comparison to the C and R groups. α-1B glycoprotein and PGD2 levels were significantly higher in the urine protein pool of the M and R groups in comparison to the C group. Annexin A1 was downregulated significantly in the urine protein pool of the M group in comparison to the C group.
Conclusion: Intensities of MASP-2 and AMBP proteins discriminated manic episode from remission period and healthy controls indicating that these proteins may be candidate biomarkers for manic episode. The decrease in Annexin A1 and increase in IgG-1 chain levels appeared to be associated with “Manic Episode” while the increase in PGD2 and α-1B glycoprotein levels appeared to be associated with “Bipolar Disorder”.
Keywords: Bipolar Disorder, Manic Episode, Urinary Proteomics, MASP-2, AMBP


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